COMPARISON OF PARASITOLOGICAL, SEROLOGICAL AND MOLECULAR DIAGNOSTIC TESTS FOR DETECTION OF SUBCLINICAL TRYPANOSOMOSIS IN TWO ORGANISED DAIRY HERDS

被引:0
|
作者
Charaya, Gaurav [1 ]
Rakha, Naresh Kumar [1 ]
Kumar, Aman [2 ]
Kumar, Akhil [3 ]
Maan, Sushila [2 ]
Singh, Ajit [3 ]
Goel, Parveen [4 ]
机构
[1] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Vet Med, Hisar, Haryana, India
[2] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Anim Biotechnol, Hisar, Haryana, India
[3] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Vet Microbiol, Hisar, Haryana, India
[4] Lala Lajpat Rai Univ Vet & Anim Sci, Hisar, Haryana, India
来源
BUFFALO BULLETIN | 2020年 / 41卷 / 02期
关键词
Bubalus bubalis; buffaloes; Trypanosomosis; diagnosis; subclinical infection; TE-LAT; PCR assay;
D O I
暂无
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Trypanosomosis also known as 'Surra' caused by Trypanosoma evansi is of significant economic importance in livestock production. Diagnosis of subclinical infection and carrier state of disease is far difficult. Therefore, the present study was conducted to compare parasitological, serological and molecular diagnostic tests for detecting subclinical trypanosomosis in two dairy herds of Murrah buffalo (n=27) and crossbred cattle (n=22) in Hisar district of Haryana. Peripheral blood samples collected from all the animals were subjected to blood smear examination and DNA isolation for molecular detection by PCR, and like-wise serum samples were collected and subjected to latex agglutination test (TE-LAT) for T. evansi antigen detection. PCR products from positive samples were purified and sequenced for confirmation of T. evansi DNA. None of the animals in the two herds was found positive for T. evansi parasites by microscopic examination of thick blood smears. In buffalo farm, 29.62% samples each were found moderately and weak positive by TE-LAT, whereas PCR assay test yielded 40.74% samples positive for T. evansi DNA. In cattle farm, TE-LAT showed 18.18%, 31.89% and 9.09% samples to be strong, moderate and weak positive, whereas 50% samples were found PCR-positive for T. evansi DNA. Agreement among TE-LAT and PCR assay, as expressed by the kappa value, was found to be poor. To conclude, each test has its own merits and limitations. While TE-LAT is suitable for screening purpose, PCR assay confirms parasite in blood. For clinical diagnosis, results of laboratory diagnostic tests must, however, be correlated clinically before initiating the therapy.
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页码:241 / 249
页数:9
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  • [1] Evaluation of serological diagnostic tests for bovine brucellosis in dairy cattle herds in an endemic area: a multicenter study
    Alamian, Saeed
    Amiry, Karim
    Bahreinipour, Akram
    Etemadi, Afshar
    Yousefi, Ali Reza
    Dadar, Maryam
    [J]. TROPICAL ANIMAL HEALTH AND PRODUCTION, 2023, 55 (02)
  • [2] Evaluation of serological diagnostic tests for bovine brucellosis in dairy cattle herds in an endemic area: a multicenter study
    Saeed Alamian
    Karim Amiry
    Akram Bahreinipour
    Afshar Etemadi
    Ali Reza Yousefi
    Maryam Dadar
    [J]. Tropical Animal Health and Production, 2023, 55
  • [3] Serological and molecular detection of Mycobacterium avium subsp paratuberculosis in cattle of dairy herds in Colombia
    Arturo Fernandez-Silva, Jorge
    Abdulmawjood, Amir
    Akineden, Oemer
    Buelte, Michael
    [J]. TROPICAL ANIMAL HEALTH AND PRODUCTION, 2011, 43 (08) : 1501 - 1507
  • [4] COMPARISON OF DIFFERENT DIAGNOSTIC TESTS FOR THE DETECTION OF SUBCLINICAL MASTITIS IN BUFFALOES
    Kala, Salikineedi Rupa
    Rani, Nalluri Lakshmi
    Rao, Velamala Vaikunta
    Subramanyam, Kothapalli Venkata
    [J]. BUFFALO BULLETIN, 2021, 40 (04): : 653 - 659
  • [5] Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia
    Jorge Arturo Fernández-Silva
    Amir Abdulmawjood
    Ömer Akineden
    Michael Bülte
    [J]. Tropical Animal Health and Production, 2011, 43 : 1501 - 1507
  • [6] Comparison of serological tests for detection of Brucella antibodies in cattle of an organized dairy farm
    Kushwaha, Neelam
    Rajora, V. S.
    Mohan, Anand
    Upadhyay, A. K.
    Kumar, Ramesh
    [J]. INDIAN JOURNAL OF ANIMAL RESEARCH, 2016, 50 (01) : 69 - 74
  • [7] Comparison of two commercial molecular tests for the detection of Clostridium difficile in the routine diagnostic laboratory
    Zidaric, Valerija
    Kevorkijan, Bozena Kotnik
    Oresic, Nadja
    Janezic, Sandra
    Rupnik, Maja
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (08) : 1131 - 1136
  • [8] Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus
    Peixoto, Rodolfo de Moraes
    Peixoto Araujo, Renata de Moraes
    Peixoto, Luciana Jatoba e Silva
    Reges, Ariel Marques
    Pereira Alves, Ana Paula
    Pinheiro Junior, Jose Wilton
    Mota, Rinaldo Aparecido
    Azevedo, Sergio Santos
    da Costa, Mateus Matiuzzi
    [J]. CIENCIA RURAL, 2016, 46 (07): : 1217 - 1222
  • [9] Comparison of serological tests for the detection of two soilborne sugar beet viruses
    Heidel, GB
    Rush, CM
    [J]. AMERICAN SOCIETY OF SUGAR BEET TECHNOLOGISTS, PROCEEDINGS, 1999, : 199 - 203
  • [10] Comparison of cow-side diagnostic tests for subclinical mastitis of dairy cows in Musanze district, Rwanda
    Iraguha, Blaise
    Hamudikuwanda, Humphrey
    Mushonga, Borden
    Kandiwa, Erick
    Mpatswenumugabo, Jean P.
    [J]. JOURNAL OF THE SOUTH AFRICAN VETERINARY ASSOCIATION, 2017, 88