Application of liguid isoelectric focusing combined acetylation of stable-isotope labeling technique in comparative proteomics

被引：0
|
作者：
Li Na [1 ]
Yang Peng-Yuan [1 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2008年 / 36卷 / 04期
关键词：
liquid isoelectric focusing; SDSPAGE; acetylation labeling; proteomics;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new method of quantitative proteomics which combines free solution-isoelectric focusing, SDSPAGE and acetylation of stable-isotope labeling techniques was developed. This technology has good dynamic range between 1:5 similar to 5:1 (less than 20%). Compared to the traditional comparative proteomics, this method not only improves the throughput of comparative proteomics but also help to find out the differentially expressed proteins in the low abundance and alkaline proteins. This technology have been applied to investigate the comparative proteomics of human liver cancer. 16 differentially expressed proteins were identified; some of these proteins have been reported to be related with liver cancer.
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页码：449 / 453
页数：5
相关论文
共 23 条
  • [1] ANN WB, 2002, ANAL BIOCHEM, V301, P161
  • [2] Strategies for the enrichment and identification of basic proteins in proteome projects
    Bae, SH
    Harris, AG
    Hains, PG
    Chen, H
    Garfin, DE
    Hazell, SL
    Paik, YK
    Walsh, BJ
    Cordwell, SJ
    [J]. PROTEOMICS, 2003, 3 (05) : 569 - 579
  • [3] CHEN ZC, 2002, CANC PROTEOMICS, P24
  • [4] The influence of epidermal growth factor receptor and tumor differentiation on the response to accelerated radiotherapy of squamous cell carcinomas of the head and neck in the randomized DAHANCA 6 and 7 study
    Eriksen, JG
    Steiniche, T
    Overgaard, J
    [J]. RADIOTHERAPY AND ONCOLOGY, 2005, 74 (02) : 93 - 100
  • [5] A two-dimensional liquid-phase separation method coupled with mass spectrometry for proteomic studies of breast cancer and biomarker identification
    Hamler, RL
    Zhu, K
    Buchanani, NS
    Kreunin, P
    Kachman, MT
    Miller, FR
    Lubman, DM
    [J]. PROTEOMICS, 2004, 4 (03) : 562 - 577
  • [6] Low-molecular-weight human serum proteome using ultrafiltration, isoelectric focusing, and mass spectrometry
    Harper, RG
    Workman, SR
    Schuetzner, S
    Timperman, AT
    Sutton, JN
    [J]. ELECTROPHORESIS, 2004, 25 (09) : 1299 - 1306
  • [7] Development of a two-dimensional protein-peptide separation protocol for comprehensive proteome measurements
    Janini, GM
    Conrads, TP
    Veenstra, TD
    Issaq, HJ
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 787 (01): : 43 - 51
  • [8] KRUGER NJ, 1996, BRADFORD METHOD PROT, P15
  • [9] PURIFICATION AND CHARACTERIZATION OF THE CORTICOSTEROID 11 BETA-DEHYDROGENASE COMPONENT OF THE RAT-LIVER 11 BETA-HYDROXYSTEROID DEHYDROGENASE COMPLEX
    LAKSHMI, V
    MONDER, C
    [J]. ENDOCRINOLOGY, 1988, 123 (05) : 2390 - 2398
  • [10] Size-exclusion chromatography in multidimensional separation schemes for proteome analysis
    Lecchi, P
    Gupte, AR
    Perez, RE
    Stockert, LV
    Abramson, FP
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2003, 56 (1-3): : 141 - 152
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