Lipopolysaccharide-induced glomerulonephritis develops in the absence of interferon-gamma signaling

IFN gamma is a costimulator of macrophage activation and it plays an important role as a proinflammatory cytokine by upregulation of adhesion molecules and MHC antigens. In this study we tested the role of IFN gamma in a model of endotoxin-induced glomerulonephritis. A systemic lupus-like disease was induced by injection of 50 mu g bacterial LPS twice a week for 4 weeks in wild-type and in IFN gamma receptor-deficient (IFN gamma R-/-) mice, The renal cortex was examined by immunofluorescence and by light microscopy. LPS treatment induced an increase in serum levels of IgG and anti-dsDNA antibodies, A mild glomerulonephritis was characterized morphologically, but proteinuria was not observed. The main histological features of glomerulonephritis were an increase in ICAM-1 expression, deposition of immune complexes and of complement in the glomeruli, increased mesangial matrix and mesangial hypercellularity, The number of intraglomerular leukocytes, detected by MHC class-II and LFA-1 expression increased roughly 4-fold, All those alterations took place in a similar maimer in wild-type and in IFN gamma R-/- mice, Therefore it is concluded that IFN gamma does not play an important role in the development of endotoxic glomerulonephritis.