ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS IN AN ENDANGERED SPECIES DRACAENA CAMBODIANA (LILIACEAE)

被引：4
|
作者：
Zhang, Lu [1 ,2 ,3 ]
Li, Qiao-Ming [1 ,3 ]
机构：
[1] Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Lab Plant Phylogenet & Conservat, Kunming 650223, Yunnan, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Menglun 666303, Yunnan, Peoples R China
来源：
AMERICAN JOURNAL OF BOTANY | 2010年 / 97卷 / 10期
关键词：
cross-species amplification; Dracaena; endangered species; microsatellite markers; SOFTWARE;
D O I：
10.3732/ajb.1000245
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: The development of microsatellite primers in the endangered species Dracaena cambodiana will be the foundation for genetic and conservation studies of D. cambodiana and several Dracaena species. Methods and Results: A total of 26 microsatellite markers were developed in Chinese populations of D. cambodiana, using the Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) protocol. Among them, sixteen primer pairs generated polymorphic loci (fourteen of them successfully amplified in other four Dracaena species) and ten primer pairs produced monomorphic loci. Conclusions: These microsatellite markers could be used in the further investigation of population genetics of D. cambodiana and other Dracaena species.
引用
收藏
页码：E91 / E93
页数：3
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