Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

被引：16

作者：

Liu, Yunpeng ^{[1
]}

Chan, Yan Mei ^{[1
]}

Wu, Jianhui ^{[1
,3
]}

Chen, Chen ^{[1
]}

Benesi, Alan ^{[1
]}

Hu, Jing ^{[2
]}

Wang, Yanming ^{[2
]}

Chen, Gong ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Biochem & Mol Biol, N Frear Lab 454A, University Pk, PA 16802 USA

[3] Capital Med Univ, Coll Pharmaceut Sci, Beijing 100069, Peoples R China

来源：

CHEMBIOCHEM | 2011年 / 12卷 / 05期

关键词：

fluorescence imaging; HCAII; mannose-6-phosphate; MPR; neoglycoproteins; N-glycans; MANNOSE 6-PHOSPHATE RECEPTORS; CONVERGENT SYNTHESIS; QUALITY-CONTROL; PART; PROTEIN; LIGAND; REACTIVITY; PROBES; OLIGOSACCHARIDES; SEMISYNTHESIS;

D O I：

10.1002/cbic.201000785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Red-ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N-glycan was achieved through a highly efficient late-stage phosphorylation strategy. A human carbonic anhydrase II-based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N-glycan-directed protein internalization process in cell-based assays by confocal fluorescence imaging. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：685 / 690

页数：6