Spinal cord repair with PHPMA hydrogel containing RGD peptides (NeuroGel™)

被引:150
|
作者
Woerly, S
Pinet, E
de Robertis, L
Van Diep, D
Bousmina, M
机构
[1] Univ Laval, Dept Chem Engn, Quebec City, PQ G1K 7P4, Canada
[2] Organogel Canada Ltee, Quebec City, PQ G1P 4R7, Canada
关键词
hydrogel; rheology; impedance spectroscopy; RGD peptide; regeneration; spinal cord;
D O I
10.1016/S0142-9612(00)00354-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A biocompatible hydrogel of poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) which includes the cell-adhesive region of fibronectin Arg-Gly-Asp was synthesized and its structure, theological and dielectric properties were characterized. The ability of a PHPMA-RGD hydrogel to promote tissue regeneration and support axonal outgrowth in the injured adult and developing rat spinal cord was evaluated. The structure of the PHPMA-RGD hydrogel displayed an interconnected porous structure, with viscoelastic properties similar to those of the neural tissue, and conductivity properties due to a peptide group. The polymer hydrogel provided a structural, three-dimensional continuity across the defect, facilitating the migration and reorganization of local wound-repair cells, as well as tissue development within the lesion. Angiogenesis and axonal growth also occurred within the microstructure of the tissue network, and supraspinal axons migrated into the reconstructed cord segment. In addition, the hydrogel induced a reduction of necrosis and cavitation in the adjacent white and gray matter. These polymer hydrogel matrices therefore display the potential to repair. tissue defects in the central nervous system by enhancing the development of a tissue equivalent as well as axonal growth across the reconstructed lesion. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1095 / 1111
页数:17
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