Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes

被引:10
|
作者
Zhang, Yang [1 ,2 ]
Wang, Lifei [1 ,2 ]
Bai, Liping [1 ,2 ]
Jiang, Rong [1 ,2 ]
Guo, Lianhong [1 ,2 ]
Wu, Jianbo [1 ,2 ]
Cheng, Guifang [2 ,3 ]
Zhang, Ren [4 ]
Li, Yuan [1 ,2 ]
机构
[1] Chinese Acad Med Sci, Inst Med Biotechnol, Key Lab Biotechnol Antibiot, Minist Hlth, Beijing 100050, Peoples R China
[2] Peking Union Med Coll, Beijing 100050, Peoples R China
[3] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
[4] Univ Wollongong, Sch Biol Sci, Wollongong, NSW 2522, Australia
关键词
NF-KAPPA-B; COLLAGEN-INDUCED ARTHRITIS; MAPK SIGNALING PATHWAYS; RHEUMATOID-ARTHRITIS; SYNOVIAL FIBROBLASTS; THERAPEUTIC TARGET; LACTOCOCCUS-LACTIS; SUGAR NUCLEOTIDE; DNA-BINDING; IN-VITRO;
D O I
10.1038/cmi.2015.36
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interleukin-1 beta-mitogen-activated protein kinase (MAPK) and NF-kappa B signaling pathways are involved in the pathogenesis of rheumatoid arthritis. Ebosin, a novel exopolysaccharide (EPS), exhibits anti-inflammatory activity in rat collagen-induced arthritis by suppressing the production of tumor necrosis factor-alpha, interleukin-6 and interleukin-1 beta. The aim of the present study was to assess the effects of ebosin on NF-kappa B and MAPK signaling pathways mediated through interleukin-1 beta in rat fibroblast-like synoviocytes (FLSs). Western blotting showed decreased production of phosphorylated p38, JNK1, JNK2, IKK alpha, IKK beta and I kappa B in the cytoplasm and NF-kappa B in the nucleus upon ebosin treatment. The DNA-binding activity of NF-kappa B in the cell nucleus was also inhibited by ebosin treatment, as demonstrated using an electrophoresis mobility gel shift assay. Analysis of the results of the immunofluorescence assay also showed a reduced amount of NF-kappa B in the nucleus of cells affected by ebosin. These results provided evidence for the effects of ebosin on both interleukin-1 beta-mediated MAPK and NF-kappa B signaling pathways in rat FLSs. In addition, enzyme-linked immunosorbent assay demonstrated that ebosin reduces the levels of matrix metalloproteinases MMP-1 and MMP-3 and the chemokines, interleukin-8 and RANTES. Thus, the results of the present study provide further evidence for understanding the medicinal activity of ebosin at a molecular level, therefore nominating this EPS as a potential therapeutic candidate for the treatment of rheumatic arthritis.
引用
收藏
页码:584 / 592
页数:9
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