Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

被引：7

作者：

Rodionov, Andrey ^{[1
]}

Deniskova, Tatiana ^{[1
]}

Dotsev, Arsen ^{[1
]}

Volkova, Valeria ^{[1
]}

Petrov, Sergey ^{[1
]}

Kharzinova, Veronika ^{[1
]}

Koshkina, Olga ^{[1
]}

Abdelmanova, Alexandra ^{[1
]}

Solovieva, Anastasia ^{[1
]}

Shakhin, Alexey ^{[1
]}

Bardukov, Nikolay ^{[1
]}

Zinovieva, Natalia ^{[1
]}

机构：

[1] LK Ernst Fed Res Ctr Anim Husb, Moscow 142132, Moscow Region, Russia

来源：

ANIMALS | 2021年 / 11卷 / 12期

基金：

俄罗斯科学基金会;

关键词：

poaching; wild goat; single-nucleotide polymorphisms; microsatellites; evidence; DNA typing; DNA;

D O I：

10.3390/ani11123416

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Simple Summary DNA molecular techniques, including multiple microsatellite analysis and genome-wide SNP-genotyping, were used to unlock and prove the poaching of wild goats (Capra caucasica) in an area of the Caucasian mountains in Russia. Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.

引用

页数：12