Competitive adsorption of lysozyme and myoglobin on mesostructured cellular foam silica

被引:4
|
作者
Darwish, Amina [1 ]
Robie, Taylor [1 ]
Desch, Rebecca J. [1 ]
Thiel, Stephen W. [1 ]
机构
[1] Univ Cincinnati, Dept Biomed Chem & Environm Engn, Cincinnati, OH 45221 USA
关键词
Adsorption model; Guggenheim-Anderson-deBoer (GAB); Multicomponent; Lysozyme; Myoglobin; PERFORMANCE LIQUID-CHROMATOGRAPHY; ORDERED MESOPOROUS MATERIALS; ISOTHERM; ENERGETICS; SYSTEMS; BIOMOLECULES; PERSPECTIVE; CONSTANTS; LANGMUIR; COLUMNS;
D O I
10.1016/j.micromeso.2015.02.010
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A model of multicomponent multilayer adsorption has been developed to describe the simultaneous adsorption of lysozyme (14.3 kDa, pI = 11.35) and myoglobin (17.6 kDa, pI = 7.2) on mesostructured cellular foam (MCF) silica in 0.02 M potassium phosphate buffer at pH 7 and 25 degrees C. The mechanism of adsorption was proposed based on batch adsorption isotherms and flow microcalorimetry (FMC) traces for the enthalpy of adsorption. Thermodynamic derivations of Guggenheim-Anderson-deBoer (GAB) and Langmuir models were used to develop the model based on the proposed mechanism. Regression analysis demonstrated the effect of each of the proposed mechanistic equations. (c) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:101 / 109
页数:9
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