Detection of invasive infection caused by Fusarium solani and non-Fusarium solani species using a duplex quantitative PCR-based assay in a murine model of fusariosis

被引：24

作者：

Bernal-Martinez, Leticia ^{[1
]}

Buitrago, Maria J. ^{[1
]}

Castelli, Maria V. ^{[1
]}

Rodriguez-Tudela, Juan L. ^{[1
]}

Cuenca-Estrella, Manuel ^{[1
]}

机构：

[1] Inst Salud Carlos III, Serv Micol, Ctr Nacl Microbiol, Madrid, Spain

来源：

MEDICAL MYCOLOGY | 2012年 / 50卷 / 03期

关键词：

Early diagnosis; emerging pathogens; DNA detection; IN-VITRO; ALKALINE PROTEASE; ANTIFUNGAL AGENTS; FUNGAL PATHOGENS; RAPID DETECTION; ASPERGILLUS; IDENTIFICATION; DIAGNOSIS; MYCOLOGY; COMPLEX;

D O I：

10.3109/13693786.2011.604047

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A duplex Real Time PCR (RT-PCR) assay for detecting DNA of members of the genus Fusarium has been developed and validated by using two mouse models of invasive infection. The duplex RT-PCR technique employed two specific molecular beacon probes targeting a highly conserved region of the fungal rDNA gene. This technique showed a detection limit of 10 fg DNA per mu l of sample and a specificity of 100%. The sensitivity in a total of 48 samples from a murine model of Fusarium solani infection was 93.9% for lung tissues and 86.7% for serum samples. In comparison, the sensitivity in a total of 45 samples of a F. oxysporum murine model infection was 87% for lung tissues and 42.8% for serum samples. This molecular technique could be a reliable method for the quantification and the evaluation of the disease in animal models and for the clinical diagnosis of fusariosis.

引用

页码：270 / 275

页数：6

共 5 条

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