Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR

被引：83
|
作者：
Wadowsky, RM
Laus, S
Green, M
Webber, SA
Rowe, D
机构：
[1] Childrens Hosp Pittsburgh, Dept Pathol, Pittsburgh, PA 15213 USA
[2] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Infect Dis & Microbiol, Pittsburgh, PA 15213 USA
[3] Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15213 USA
[4] Univ Pittsburgh, Sch Med, Dept Pediat, Pittsburgh, PA 15213 USA
[5] Univ Pittsburgh, Sch Med, Dept Surg, Pittsburgh, PA 15213 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 11期
关键词：
D O I：
10.1128/JCM.41.11.5245-5249.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0.900), whereas the plasma and PBL loads correlated poorly (r(2) = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.
引用
收藏
页码：5245 / 5249
页数：5
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