FTIR spectra of grapevines (Vitis vinifera L.) in the presence of virus infections

被引：2
|
作者：
Topala, C. M. [1 ]
Tataru, L. D. [1 ]
Buciumeanu, E. C. [2 ]
Guta, I. C. [2 ]
机构：
[1] Univ Pitesti, Fac Sci, Dept Nat Sci, Pitesti, Romania
[2] Natl Res & Dev Inst Biotechnol Hort Stefanesti Ar, Stefanesti, Romania
来源：
X INTERNATIONAL SYMPOSIUM ON GRAPEVINE PHYSIOLOGY AND BIOTECHNOLOGY | 2017年 / 1188卷
关键词：
Vitis; FTIR spectroscopy; viral infection; INFRARED MICROSPECTROSCOPY; 1ST REPORT;
D O I：
10.17660/ActaHortic.2017.1188.41
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A nondestructive, efficient and accurate fingerprinting method using Fourier Transform Infrared spectroscopy is proposed for diagnosing virus infection in grapevine plants. This analysis explores the vibration of functional groups present in macromolecules and shows the molecular structural changes through shifts in wavenumber. Three viruses were used on six grapevine cultivars: Grapevine fleck virus - infected 'Moldova' and 'Grasa de Cotnari', Grapevine fanleaf virus - infected 'Feteasca neagra 7 Od.' and 'Italia', Grapevine leafroll associated virus type 1+3 - infected 'Cabernet Sauvignon' and 'Bicane'. Symptom-free leaves from healthy and virus infected grapevines were used. The virus infected grapevine plants revealed signs of change in the FTIR spectra. The presence of virus-induced disease in grapevine plants showed a distinctive spectrum in carbohydrates (1185-1000 cm(-1)) that could be used to distinguish an infected plant from a healthy one. Leafroll associated virus type 1+3 induced the most evident spectrum changes in infected plants.
引用
收藏
页码：313 / 318
页数：6
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