Preparation and characterization on Cecropin B peptide grafted with silk fibroin films

The undisolvable Bombyx mori silk fibroin film was prepared from the regenerated silk fibroin solution followed with the 60% ( volume fraction) ethanol aqueous solution treatment. The silk fibroin film was grafted with Cecropin B peptide via the carbodiimide chemistry. The secondary structure, the film surface properties and the bacteriostatic activity of the obtained silk fibroin film were analyzed with FTIR, SEM, EDS and the antibacterial tests. The results show that the Cecropin B peptide is covalently grafted onto the surface of silk fibroin film, which is undisolvable and has an effective and permanent bacteriostatic activity.