D-aspartate and N-methyl-D-aspartate promote proliferative activity in mouse spermatocyte GC-2 cells

被引:12
|
作者
Falvo, Sara [1 ]
Santillo, Alessandra [1 ]
Baccari, Gabriella Chieffi [1 ]
Cioffi, Federica [2 ]
Di Fiore, Maria Maddalena [1 ]
机构
[1] Univ Campania Luigi Vanvitelli, Dipartimento Sci & Tecnol Ambientali Biol & Farma, Caserta, Italy
[2] Univ Sannio, Dipartimento Sci & Tecnol, Benevento, Italy
关键词
D-aspartate; N-methyl-D-aspartate; GC-2; cells; Spermatocytes; Mitochondria; Mitochondrial fusion; Mitochondrial fission; Mitochondrial biogenesis; NUCLEAR RESPIRATORY FACTORS; ACTIVATED PROTEIN-KINASES; MITOCHONDRIAL BIOGENESIS; METABOLIC REQUIREMENTS; TRANSCRIPTION FACTOR; HARDERIAN-GLAND; RANA-ESCULENTA; ACID; FROG; EXPRESSION;
D O I
10.1016/j.repbio.2021.100601
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
D-Aspartate (D-Asp) and its methylated form N-methyl-D-aspartate (NMDA) promote spermatogenesis by stimulating the biosynthesis of sex steroid hormones. D-Asp also induces spermatogonia proliferation directly by activating the ERK/Aurora B pathway. In the present study, a mouse spermatocyte-derived cell line (GC-2) which represents a stage between preleptotene spermatocyte and round spermatids was exposed to 200 mu M D-Asp or 50 mu M NMDA for 30 min, 2 h, and 4 h to explore the influence of these amino acids on cell proliferation and mitochondrial activities occurring during this process. By Western blotting analyses, the expressions of AMPAR (GluAl-GluA2/3 subunits), cell proliferation as well as mitochondria functionality markers were determined at different incubation times. The results revealed that D-Asp or NMDA stimulate proliferation and meiosis in the GC-2 cells via the AMPAR/ERK/Akt pathway, which led to increased levels of the PCNA, p-H3, and SYCP3 proteins. The effects of D-Asp and NMDA on the mitochondrial functionality of the GC-2 cells strongly suggested an active role of these amino acids in germ cell maturation. In both D-Asp- and NMDA-treated GC-2 cells mitochondrial biogenesis as well as mitochondrial fusion are increased while mitochondria fission is inhibited. Finally, the findings showed that NMDA significantly increased the expressions of the CII, CIII, CIV, and CV complexes of oxidative phosphorylation system (OXPHOS), whereas D-Asp induced a significant increase in the expressions only of the CIV and CV complexes. The present study provides novel insights into the mechanisms underlying the role of D-Asp and NMDA in promoting spermatogenesis. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] Occurrence of D-aspartate and N-methyl-D-aspartate in the serum, liver and brain of chicken
    Poisson, JJ
    Mirza, RA
    Ferrandino, G
    Spinelli, P
    D'Aniello, A
    Fisher, G
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 229 : U112 - U112
  • [2] Determination of D-aspartate and N-methyl-D-aspartate in the ring ganglia of aplysia and their involvement in learning and memory
    Peterson, K
    Christian, A
    Rios, J
    Fisher, G
    D'Aniello, A
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2002, 223 : U236 - U236
  • [3] ADMINISTRATION OF D-ASPARTATE INCREASES D-ASPARTATE OXIDASE ACTIVITY IN MOUSE-LIVER
    YAMADA, R
    NAGASAKI, H
    NAGATA, Y
    WAKABAYASHI, Y
    IWASHIMA, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 990 (03) : 325 - 328
  • [4] D-Aspartate Induces Proliferative Pathways in Spermatogonial GC-1 Cells
    Santillo, Alessandra
    Falvo, Sara
    Chieffi, Paolo
    Di Fiore, Maria Maddalena
    Senese, Rosalba
    Baccari, Gabriella Chieffi
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2016, 231 (02) : 490 - 495
  • [5] Determination of D-aspartate N-methyltransferase activity in the starfish by direct analysis of N-methyl-D-aspartate with high-performance liquid chromatography
    Shibata, Kimihiko
    Sugaya, Noriko
    Ono, Wakana
    Abe, Katsumasa
    Takahashi, Shouji
    Kera, Yoshio
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (29): : 3229 - 3234
  • [6] Ncm-D-aspartate:: A novel caged D-aspartate suitable for activation of glutamate transporters and N-methyl-D-aspartate (NMDA) receptors in brain tissue
    Huang, YHH
    Muralidharan, S
    Sinha, SR
    Kao, JPY
    Bergles, DE
    [J]. NEUROPHARMACOLOGY, 2005, 49 (06) : 831 - 842
  • [7] Protein kinase C potentiation of N-methyl-D-aspartate receptor activity is not mediated by phosphorylation of N-methyl-D-aspartate receptor subunits
    Zheng, X
    Zhang, L
    Wang, AP
    Bennett, MVL
    Zukin, RS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) : 15262 - 15267
  • [8] Neuroprotection by N-methyl-d-aspartate antagonists
    Himmelseher, S.
    Kochs, E. F.
    [J]. ANAESTHESIA, PAIN, INTENSIVE CARE AND EMERGENCY MEDICINE, 2005, : 627 - 632
  • [9] N-methyl-D-aspartate receptors in the retina
    Shen, Yin
    Liu, Xiao-Ling
    Yang, Xiong-Li
    [J]. MOLECULAR NEUROBIOLOGY, 2006, 34 (03) : 163 - 179
  • [10] N-Methyl-d-aspartate receptors in the retina
    Yin Shen
    Xiao-Ling Liu
    Xiong-Li Yang
    [J]. Molecular Neurobiology, 2006, 34 : 163 - 179