Chemical Constituents from Nicotiana tabacum L. and Their Antifungal Activity

A phytochemical investigation of an aqueous ethyl alcohol (EtOH) extract of the flowers of Nicotiana tabacum L. led to the identification of one new eudesmane-type sesquiterpenoid (1) and 14 known compounds, including 7 flavones (2-8), 6 phenolic acids (9-14), and 1 coumarin (15). The chemical structure of 1 was established on the basis of high-resolution electrospray ionization (HRESIMS) and 1D and 2D NMR analyses, while the other structures were elucidated by 1D NMR and LC-MS data. The antiphytopathogenic activities of all the isolated compounds were tested to determine their antifungal activity against Valsa mali var. mali, Alternaria porri, and Botrytis cinerea at a concentration of 10 mu g/mL. Compound 4 exhibited more potent antifungal effects against Valsa mali var. mali with a % growth inhibition of 47.9 +/- 1.2%, while the others only showed insignificant activities.