Efficient multiple-solvent suppression for the study of the interactions of organic solvents with biomolecules

被引：124
|
作者：
Dalvit, C [1 ]
机构：
[1] Novartis Pharma AG, CH-4002 Basel, Switzerland
来源：
JOURNAL OF BIOMOLECULAR NMR | 1998年 / 11卷 / 04期
关键词：
excitation sculpting; hydration; multiple-solvents suppression; organic solvents; pulsed field gradients;
D O I：
10.1023/A:1008272928075
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A modification of the excitation sculpting sequence [Hwang, T.-L. and Shaka, A.J. (1995) J. Magn. Reson., A112, 275-279] for achieving efficient multiple-solvents suppression in 1D and 2D H-1 NMR experiments is presented. Implementation of this scheme in the ePHOGSY experiment allows rapid and sensitive detection of weak interactions between organic solvents or small molecules and biomolecules. Application of the techniques to the peptide Sandostatin (R) dissolved in H2O and DMSO is demonstrated.
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页码：437 / 444
页数：8
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