Agrococcus terreus sp nov and Micrococcus terreus sp nov., isolated from forest soil

Two bacterial strains, DNG5(T) and V3M1(T), isolated from forest soil of the Changbai mountains in China, were characterized using a polyphasic approach. Analysis of their 16S rRNA gene sequences indicated that strains DNG5(T) and V3M1(T) were phylogenetically related to members of the genus Agrococcus (96.0-98.4% similarity) and Micrococcus (96.7-98.0 % similarity), respectively, within the order Actinomycetales. Strains DNG5T and V3M1T were Gram-stain-positive and strictly aerobic and formed yellow colonies on LB agar. Cells of strain DNG5T were short, non-motile rods, 0.4-0.5x0.8-1.0 mu m. Strain DNG5T contained MK-10 and MK-11 as the major respiratory quinones and anteiso-(15:0) (49.2%) and iso-C-16:0 (22.4%) as the major fatty acids. The diamino acid in the peptidoglycan of strain DNG5T was 2,4-diaminobutyric acid and the murein was of the acetyl type. Cells of strain V3M1T were cocci, 0.6-0.7 mu m in diameter. The cell-wall peptidoglycan of strain V3M1T contained the amino acids lysine, glutamic acid, alanine and glycine. Strain V3M1T contained MK-7, MK-7(H-2), MK-8 and MK-8(H-2) as respiratory quinones and anteiso-C-15:0 (78.2%) and iso-C-15:0 (13.1%) as the major cellular fatty acids. The DNA G+C contents of strains DNG5(T) and V3M1(T) were 75.9 and 67.2 mol%, respectively. The DNA DNA relatedness of strain DNG5(T) to Agrococcus jejuensis DSM 22002(T), A. jenensis JCM 9950(T), A. baldri JCM 12132(T) and A. citreus JCM 12398(T) was 58.3, 43.9, 36.1 and 54.1%, respectively. The DNA DNA relatedness of strain V3M1T to Micrococcus luteus CGMCC 1.2299(T), M. antarcticus CGMCC 1.2373(T) and M. lylae CGMCC 1.2300(T) was 57.5, 45.4 and 39.00/o, respectively. Combining phenotypic and genotypic traits, strain DNG5T represents a novel species of the genus Agrococcus, for which the name Agrococcus terreus sp. nov. is proposed, with DNG5T (=CGMCC 1.6960(T) =NBRC 104260(T)) as the type strain. Strain V3M1T represents a novel species of the genus Micrococcus, for which the name Micrococcus terreus sp. nov. is proposed, with V3M1T (= CGMCC 1.7054(T) =NBRC 104258(T)) as the type strain.