The research of miR-301 in gastric cancer cell proliferation and apoptosis

We investigated the impact and mechanisms of miR-301 in gastric cancer cell proliferation and apoptosis, and looked forward to find a new frame of reference in the clinical diagnosis and treatment. 100 case gastric carcinoma collected from our hospital gastrointestinal surgery were as the observation group (group A); then collected 100 cases of carcinoma adjacent as the negative control group (group B) and 10 healthy adult normal gastric cell line (GES-1) as the healthy control group (group C). Used real-time PCR to analyze the miR-301 expression of the three groups, MTT assay detect cell proliferation, annexin V/PI staining detect cell apoptosis, TargetScans software predict target genes, then used luciferase reporter gene assay to verify the predicted target genes. Western blot was used to detect the predicted miR-301 target gene expression. The results showed that miR-301 expression from the observation group was significantly higher than the negative control group and the healthy control group (P < 0.05). The absorbance values measured showed that the observation group was significantly higher than the other two groups (P < 0.05). After transfection anti-miR-301, MGC-803 apoptosis index was significantly higher than (NC) group and control (Ctr) group (P < 0.05). MiR-301 expression was positively correlated with tumor size, TNM staging and Lauren type, and it had no correlation with gender, age, lymph node metastasis and tissue differentiation. Targetsscan software predicted the target gene of miR-301 may be PTEN, which was confirmed by luciferase reporter assay. Western blot showed that transfection of miR-301 mimic significantly inhibited the expression of PTEN in MGC-803 cells (P < 0.01), whereas transfection anti-miR-301 could significantly increase the PTEN expression (P < 0.01). MiR-301 upregulated in gastric cancer, high expression of miR-301 may be involved in the occurrence and development of gastric cancer by adjusting the target gene PTEN.