The engineering of decamericd-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions

被引：8

作者：

Yang, Xiaohong ^{[1
,2
]}

Wu, Lian ^{[3
,4
,5
]}

Li, Aipeng ^{[1
]}

Ye, Lidan ^{[1
,2
]}

Zhou, Jiahai ^{[3
]}

Yu, Hongwei ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Coll Chem & Biol Engn, Key Lab Biomass Chem Engn, Educ Minist, Hangzhou 310027, Peoples R China

[2] Zhejiang Univ, Inst Bioengn, Coll Chem & Biol Engn, Hangzhou 310027, Peoples R China

[3] Univ Chinese Acad Sci, Ctr Excellence Mol Synth, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China

[4] Chinese Acad Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

[5] Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 55期

关键词：

D-FRUCTOSE-6-PHOSPHATE ALDOLASE; FRUCTOSE-6-PHOSPHATE ALDOLASE; ESCHERICHIA-COLI; TRANSALDOLASE; STABILITY;

D O I：

10.1039/d0cc02437f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The combinatorial modulation of inter- and intra-subunit interactions of decamericd-fructose-6-phosphate aldolase A (FSAA) generated a triple-site variant I31T/Q59T/I195Q FSAA with 27- to 278-fold improvement in activity towards target heteroaromatic aldehydes. X-ray crystallographic data and molecular dynamics simulations ascribed the enhanced activity to the pronounced flexibility of the interface region between subunits, the expanded substrate entrance and binding pocket, and enhanced proton transfer, unambiguously demonstrating the efficiency of this strategy for engineering multimeric enzymes.

引用

页码：7561 / 7564

页数：4