Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of Sao Paulo, Brazil

被引：25
|
作者：
Thomaz, Alexandre [1 ]
Meireles, Marcelo V. [2 ]
Soares, Rodrigo M. [1 ]
Pena, Hilda F. J. [1 ]
Gennari, Solange M. [1 ]
机构：
[1] Univ Sao Paulo, Fac Med Vet Zootecn, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, Brazil
[2] Univ Estadual Paulista, Dept Clin Cirurg & Reprod Anim, Aracatuba, Brazil
来源：
VETERINARY PARASITOLOGY | 2007年 / 150卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
Cryptosporidium; genotypic characterization; cats; dogs; bovines;
D O I：
10.1016/j.vetpar.2007.09.023
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The objective of this study was to obtain information of epidemiological nature through genotypic characterization of Cryptosporidium isolates from dogs, cats and bovines from the state of Sao Paulo, Brazil. The extraction of DNA from oocysts was carried out and polymerase chain reaction was accomplished using specific primers to 18S rRNA gene. The amplicons were directed sequenced. Seven cat samples, nine dog samples and nine bovine samples were analysed. From the seven cat samples the genotypic analyses revealed Cryptosporidium felis in all. These were the first genotypic characterization of Cryptosporidium from domestic felines in Brazil. In nine sequenced samples from dogs, genotypic identities compatible with Cryptosporidium canis were revealed in all samples. The genotypic analyses in bovines revealed Cryptosporidium parvum in eight samples and Cryptosporidium bovis in another sample, the last one being a non-zoonotic species, not related to clinical symptoms and described for the first time in Brazil. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：291 / 296
页数：6
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