Nucleic Acid-Based Approaches for Detection of Viral Hepatitis

被引:20
|
作者
Behzadi, Payam [1 ]
Ranjbar, Reza [1 ]
Alavian, Seyed Moayed [2 ]
机构
[1] Baqiyatallah Univ Med Sci, Mol Biol Res Ctr, Tehran, Iran
[2] Baqiyatallah Univ Med Sci, Res Ctr Gastroenterol & Liver Dis, Tehran, Iran
关键词
Nucleic Acids; Molecular Diagnostic Techniques; Hepatitis; MEDIATED ISOTHERMAL AMPLIFICATION; DNA MICROARRAY TECHNOLOGY; POLYMERASE-CHAIN-REACTION; B-VIRUS DNA; PCR; QUANTITATION; ACCURATE; MUTATION; DEVICES; HBV;
D O I
10.5812/jjm.17449
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Context: To determining suitable nucleic acid diagnostics for individual viral hepatitis agent, an extensive search using related keywords was done in major medical library and data were collected, categorized, and summarized in different sections. Results: Various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. These molecular assays are proper technologies for rapidly detecting viral agents with high accuracy, high sensitivity, and high specificity. Nonetheless, the application of each diagnostic method is completely dependent on viral agent. Conclusions: Despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages.
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页数:7
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