Mechanism of irregular firing of suprachiasmatic nucleus neurons in rat hypothalamic slices

被引:47
|
作者
Kononenko, NI [1 ]
Dudek, FE [1 ]
机构
[1] Colorado State Univ, Dept Biomed Sci, Sect Anat & Neurobiol, Ft Collins, CO 80523 USA
关键词
D O I
10.1152/jn.00314.2003
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The mechanisms of irregular firing of spontaneous action potentials in neurons from the rat suprachiasmatic nucleus (SCN) were studied in hypothalamic slices using cell-attached and whole cell recording. The firing pattern of spontaneous action potentials could be divided into regular and irregular, based on the interspike interval (ISI) histogram and the membrane potential trajectory between action potentials. Similar to previous studies, regular neurons had a firing rate about >3.5 Hz and irregular neurons typically fired about <3.5 Hz. The ISI of irregular-firing neurons was a linear function of the sum of inhibitory postsynaptic potentials (IPSPs) between action potentials. Bicuculline (10-30 μM) suppressed IPSPs and converted an irregular pattern to a more regular firing. Bicuculline also depolarized SCN neurons and induced bursting-like activity in some SCN neurons. Gabazine (20 μM), however, suppressed IPSPs without depolarization, and also converted irregular activity to regular firing. Thus GABA(A) receptor-mediated IPSPs appear responsible for irregular firing of SCN neurons in hypothalamic slices.
引用
收藏
页码:267 / 273
页数:7
相关论文
共 50 条
  • [1] SPONTANEOUS FIRING OF CULTURED NEURONS FROM THE HYPOTHALAMIC SUPRACHIASMATIC NUCLEUS (SCN) OF THE RAT
    WALSH, I
    VANDENBERG, RJ
    MARANI, E
    RIETVELD, WJ
    [J]. JOURNAL OF INTERDISCIPLINARY CYCLE RESEARCH, 1988, 19 (03): : 215 - 216
  • [2] REGULARLY FIRING NEURONS IN THE RAT SUPRACHIASMATIC NUCLEUS
    GROOS, GA
    HENDRIKS, J
    [J]. EXPERIENTIA, 1979, 35 (12): : 1597 - 1599
  • [3] Mechanism of spontaneous firing in dorsomedial suprachiasmatic nucleus neurons
    Jackson, AC
    Yao, GL
    Bean, BP
    [J]. JOURNAL OF NEUROSCIENCE, 2004, 24 (37): : 7985 - 7998
  • [4] Electrophysiological and morphological heterogeneity of neurons in slices of rat suprachiasmatic nucleus
    Pennartz, CMA
    De Jeu, MTG
    Geurtsen, AMS
    Sluiter, AA
    Hermes, MLHJ
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1998, 506 (03): : 775 - 793
  • [5] ELECTRICAL AND METABOLIC-ACTIVITY OF SUPRACHIASMATIC NUCLEUS NEURONS IN HAMSTER HYPOTHALAMIC SLICES
    SHIBATA, S
    MOORE, RY
    [J]. BRAIN RESEARCH, 1988, 438 (1-2) : 374 - 378
  • [6] Burst firing of oxytocin neurons in male rat hypothalamic slices
    Wang, YF
    Hatton, GI
    [J]. BRAIN RESEARCH, 2005, 1032 (1-2) : 36 - 43
  • [7] TEMPERATURE SENSITIVITY OF SUPRACHIASMATIC NEURONS IN RAT HYPOTHALAMIC TISSUE-SLICES
    DERAMBURE, PS
    BOULANT, JA
    [J]. FASEB JOURNAL, 1992, 6 (04): : A1199 - A1199
  • [8] Circadian difference in firing rate of isolated rat suprachiasmatic nucleus neurons
    Kononenko, Nikolai I.
    Kuehl-Kovarik, M. Cathleen
    Partin, Kathryn M.
    Dudek, F. Edward
    [J]. NEUROSCIENCE LETTERS, 2008, 436 (03) : 314 - 316
  • [9] Membrane properties and morphology of vasopressin neurons in slices of rat suprachiasmatic nucleus
    Pennartz, CMA
    Bos, NPA
    De Jeu, MTG
    Geurtsen, AMS
    Mirmiran, M
    Sluiter, AA
    Buijs, RM
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1998, 80 (05) : 2710 - 2717
  • [10] Effects of sodium pump activity on spontaneous firing in neurons of the rat Suprachiasmatic nucleus
    Wang, Yi-Chi
    Huang, Rong-Chi
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 2006, 96 (01) : 109 - 118