Amperometric glucose biosensor based on ultrafine platinum nanoparticles

In the present paper the ultrafine and highly dispersed platinum nanoparticles ( average size 3 nm) were used for the construction of a glucose biosensor in a simple method. An excellent response to glucose has been obtained with a high sensitivity (137.7 mu A mM(-1) cm(-2)) and fast response time (5 s). The biosensor showed a detection limit of 5 mM (at the ratio of signal to noise, S/ N = 3) and a linear range form 0.2 to 3.2 mM with a correlation coefficient r = 0.999. The apparent Michaelis - Menten constant (k(m)) and the maximum current were estimated to be 9.36 and 1.507 mA mM(-1) cm(-2), respectively. In addition, effects of pH value, applied potential and the interferents on the amperometric response of the sensor were investigated and discussed.