Antioxidant efficacy of (-)-loliolide isolated fromSargassum horneriagainst AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo

Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (-)-loliolide, a monoterpenoid isolated fromSargassum horneriin Vero cells and zebrafish models. (-)-Loliolide was purified from the chloroform fraction of anS. hornerimethanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (-)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (-)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 +/- 0.005 mg mL(-1)). In addition, our study showed that (-)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2 '-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (-)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (-)-loliolide. Future studies highlighting the potential applications ofS. horneriin manufacturing functional food ingredients are required.