Direct PCR detection of Escherichia coli O157:H7

被引：78
|
作者：
Fode-Vaughan, KA [1 ]
Maki, JS [1 ]
Benson, JA [1 ]
Collins, MLP [1 ]
机构：
[1] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2003年 / 37卷 / 03期
关键词：
detection; Escherichia coli O157 : H7; PCR; Shiga toxin; STEC; stx;
D O I：
10.1046/j.1472-765X.2003.01386.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC). Methods and Results: Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk. Conclusions: PCR reactions containing one cell yielded a DPCR product. Significance and Impact of the Study: This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.
引用
收藏
页码：239 / 243
页数：5
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