An Alu-based, MGB Eclipse™ real-time PCR method for quantitation of human DNA in forensic samples

被引：0

作者：

Nicklas, JA ^{[1
]}

Buel, E ^{[1
]}

机构：

[1] Vermont Forens Lab, Dept Publ Safety, Waterbury, VT 05671 USA

来源：

JOURNAL OF FORENSIC SCIENCES | 2005年 / 50卷 / 05期

关键词：

forensic science; human DNA; DNA quantitation; A/u sequences; polymerase chain reaction; real-time; MGB Eclipse (TM);

D O I：

暂无

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse (TM) primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/mu l to 0.5 pg/mu L).

引用

页码：1081 / 1090

页数：10

共 50 条

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