A homogeneous, high-throughput fluorescence resonance energy transfer-based DNA polymerase assay

被引：16
|
作者：
Shapiro, A [1 ]
Rivin, O
Gao, N
Hajec, L
机构：
[1] AstraZeneca R&D Boston, Dept Biochem, Infect Discovery, Waltham, MA 02451 USA
[2] AstraZeneca R&D Boston, Dept Mol Sci, Infect Discovery, Waltham, MA 02451 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2005年 / 347卷 / 02期
关键词：
DNA polymerase; fluorescence resonance energy transfer; FRET; DnaE; pol III; high-throughput;
D O I：
10.1016/j.ab.2005.09.023
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A homogeneous, fluorescence resonance energy transfer (FRET)-based DNA polymerase assay that is suitable for high-throughput screening for inhibitors, and can also be used for steady-state kinetic investigations, is described. The activity, kinetic mechanism, and processivity of the isolated a subunit of DNA polymerase 111, the product of the dnaE gene, from the gram-negative pathogen Haemophilus influenzae were investigated using the FRET assay. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：254 / 261
页数：8
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