Dynamic computer simulations of the influence of injection conditions on capillary zone electrophoretic stacking of preparative free-flow zone electrophoresis fractions of peptides

被引：7

作者：

Reijenga, JC

Kasicka, V

机构：

[1] Eindhoven Univ Technol, Instrumental Anal Lab, NL-5600 MB Eindhoven, Netherlands

[2] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 836卷 / 01期

关键词：

computer simulation; injection methods; stacking; peptides;

D O I：

10.1016/S0021-9673(98)00982-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynamic computer simulations were carried out to investigate the effect of residual acetic acid content on the initial stacking conditions of oligoglycine samples during the analytical CE run. These simulations were carried out with both individual peptides and with their equimolar mixtures. Results indicate that optimum acetic acid conditions were in the range of equimolar values. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：129 / 136

页数：8

共 18 条

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[6] Up-scaling capillary zone electrophoresis separations of polydisperse anionic polyelectrolytes with preparative free-flow electrophoresis exemplified with a soil fulvic acid
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ELECTROPHORESIS, 2002, 23 (17) : 2872 - 2879
[7] Theory of the correlation between capillary and free-flow zone electrophoresis and its use for the conversion of analytical capillary separations to continuous free-flow preparative processes -: Application to analysis and preparation of fragments of insulin
Kasicka, V
Prusík, Z
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JOURNAL OF CHROMATOGRAPHY A, 1998, 796 (01) : 211 - 220
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Kasicka, Vaclav
ELECTROPHORESIS, 2009, 30 : S40 - S52
[9] Theory of the correlation between capillary and free-flow zone electrophoresis and its use for the conversion of analytical capillary separations to continuous free-flow preparative processes. Application to analysis and preparation of fragments of insulin
J Chromatogr, 1 (211-220):
[10] TEMPERATURE ELEVATIONS OF THE SAMPLE ZONE IN FREE SOLUTION CAPILLARY ELECTROPHORESIS UNDER STACKING CONDITIONS
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