CHARACTERIZATION OF TEA (CAMELLIA SINENSISL.) GENOTYPES GROWN IN TURKEY BY ISSR MARKERS

被引：2
|
作者：
Yogurtcu, B. [1 ]
Aygun, A. [2 ,3 ]
机构：
[1] Ordu Univ, Dept Hort, Fac Sci, TR-52000 Ordu, Turkey
[2] Kocaeli Univ, Fac Arts & Sci, Dept Biol, TR-41380 Kocaeli, Turkey
[3] Kyrgyz Turkish Manas Univ, Dept Hort & Field Crops, Fac Agr, Bishkek 720044, Kyrgyzstan
来源：
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH | 2021年 / 19卷 / 05期
关键词：
Camellia sinensis; CTAB; endangered; Taq polymorphism; PCR; GENETIC DIVERSITY; RAPD; GERMPLASM; CHINA; POLYMORPHISMS; CULTIVARS; PLANT;
D O I：
10.15666/aeer/1905_41034114
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
The aim of this study was to determine the genetic diversity of 18 tea (Camellia sinensis) genotypes and 6 varieties collected from different provinces in the Black Sea region of Turkey. DNA isolation was performed according to the CTAB method of tea samples. PCR was performed for all DNA samples with 59 ISSR primers. In order to determine the genetic relationship between tea genotypes among these primers, 15 polymorphic ISSR primers were used. At the end of the PCR procedures, the polymorphism rate was determined as 77% from the bands formed. As a result of the analysis of products obtained by PCR studies, 109 bands were obtained in ISSR primers. The obtained bands were scored as presence (1) and absence (0) and their files were created. A genetic relation dendrogram was formed according to the obtained band results. Genetic diversity of tea genotypes and varieties of the Black Sea region was determined by molecular markers.
引用
收藏
页码：4103 / 4114
页数：12
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