Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

被引：0
|
作者：
Cingel, Aleksandar [1 ]
Vinterhalter, Branka [1 ]
Vinterhalter, Dragan [1 ]
Calic-Dragosavac, Dusica [1 ]
Smigocki, Ann [2 ]
Ninkovic, Slavica [1 ]
机构：
[1] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade 11060, Serbia
[2] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2010年 / 9卷 / 30期
关键词：
Agrobacterium tumefaciens; oryzacystatin; Solanum tuberosum L; PROTEINASE-INHIBITOR; PLANT-REGENERATION; SHOOT REGENERATION; MOLECULAR-CLONING; ORYZACYSTATIN-II; DIPLOID POTATO; EXPRESSION; RESISTANCE; GENE; LEAF;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.
引用
收藏
页码：4644 / 4650
页数：7
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