Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy

被引：1
|
作者：
Kaneshiro, Junichi [1 ]
Shioi, Go [1 ]
Okamoto, Kazuko [1 ]
Onami, Shuichi [2 ]
Watanabe, Tomonobu M. [1 ,3 ]
机构：
[1] RIKEN Ctr Biosyst Dynam Res BDR, Lab Comprehens Bioimaging, Chuo Ku, 2-2-3 Minatomachi Minami, Kobe, Hyogo 6500047, Japan
[2] RIKEN Ctr Biosyst Dynam Res BDR, Lab Dev Dynam, Chuo Ku, 2-2-3 Minatomachi Minami, Kobe, Hyogo 6500047, Japan
[3] Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Stem Cell Biol, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan
来源：
OPTICS EXPRESS | 2021年 / 29卷 / 15期
关键词：
CELL; RECONSTRUCTION;
D O I：
10.1364/OE.423783
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Multidirectional digital scanned laser light-sheet microscopy (mDSLM) cannot be used with the current pseudo confocal system to reduce blurring and background signals. The multiline scanning for light-sheet illumination and the simple image construction proposed in this study are alternative to the pseudo confocal system. We investigate the effectiveness of our pseudo confocal method combined with mDSLM on artificial phantoms and biological samples. The results indicate that image quality from mDSLM can be improved by the confocal effect; their combination is effective and can be applied to biological investigations. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：24278 / 24288
页数：11
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