Cryopreservation of bovine oocytes: Current status and recent developments

被引:44
|
作者
Massip, A [1 ]
机构
[1] Catholic Univ Louvain, Inst Sci Vie, Unite Sci Vet, B-1348 Louvain, Belgium
来源
REPRODUCTION NUTRITION DEVELOPMENT | 2003年 / 43卷 / 04期
关键词
oocyte; bovine; cryopreservation;
D O I
10.1051/rnd:2003024
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0degreesC. Therefore, cryopreservation of oocytes by vitrification - especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications - seems the most appropriate method.
引用
收藏
页码:325 / 330
页数:6
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