A novel plant gene essential for meiosis is related to the human CtIP and the yeast COM1/SAE2 gene

被引:86
|
作者
Uanschou, Clemens [1 ]
Siwiec, Tanja [1 ]
Pedrosa-Harand, Andrea [1 ]
Kerzendorfer, Claudia [1 ,2 ]
Sanchez-Moran, Eugenio [3 ]
Novatchkova, Maria [4 ]
Akimcheva, Svetlana [2 ]
Woglar, Alexander [1 ]
Klein, Franz [1 ]
Schloeglhofer, Peter [1 ]
机构
[1] Univ Vienna, Max F Perutz Lab, Dept Chromosomes Biol, A-1030 Vienna, Austria
[2] Austrian Acad Sci, Gregor Mendel Inst Mol Plant Biol, Vienna, Austria
[3] Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England
[4] Res Inst Mol Pathol, Vienna, Austria
来源
EMBO JOURNAL | 2007年 / 26卷 / 24期
基金
奥地利科学基金会;
关键词
Arabidopsis; CtIP; DNA repair; meiosis; SAE2;
D O I
10.1038/sj.emboj.7601913
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obligatory homologous recombination (HR) is required for chiasma formation and chromosome segregation in meiosis I. Meiotic HR is initiated by DNA double-strand breaks (DSBs), generated by Spo11, a homologue of the archaebacterial topoisomerase subunit Top6A. In Saccharomyces cerevisiae, Rad50, Mre11 and Com1/Sae2 are essential to process an intermediate of the cleavage reaction consisting of Spo11 covalently linked to the 50 termini of DNA. While Rad50 and Mre11 also confer genome stability to vegetative cells and are well conserved in evolution, Com1/Sae2 was believed to be fungal-specific. Here, we identify COM1/SAE2 homologues in all eukaryotic kingdoms. Arabidopsis thaliana Com1/Sae2 mutants are sterile, accumulate AtSPO11-1 during meiotic prophase and fail to form AtRAd51 foci despite the presence of unrepaired DSBs. Furthermore, DNA fragmentation in AtCom1 is suppressed by eliminating AtSPO11-1. In addition, AtCOM1 is specifically required for mitomycin C resistance. Interestingly, we identified CtIP, an essential protein interacting with the DNA repair machinery, as the mammalian homologue of Com1/Sae2, with important implications for the molecular role of CtIP.
引用
收藏
页码:5061 / 5070
页数:10
相关论文
共 50 条
  • [1] Analysis of COM1/Sae2 in meiosis and mitosis
    Knezic, I
    Klein, F
    CYTOGENETICS AND CELL GENETICS, 1999, 85 (1-2): : 34 - 34
  • [2] A conserved function for a Caenorhabditis elegans Com1/Sae2/CtIP protein homolog in meiotic recombination
    Penkner, Alexandra
    Portik-Dobos, Zsuzsanna
    Tang, Lois
    Schnabel, Ralf
    Novatchkova, Maria
    Jantsch, Verena
    Loidl, Josef
    EMBO JOURNAL, 2007, 26 (24): : 5071 - 5082
  • [3] Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae:: A role for SAE2/COM1
    Rattray, AJ
    McGill, CB
    Shafer, BK
    Strathern, JN
    GENETICS, 2001, 158 (01) : 109 - 122
  • [4] MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena
    Lukaszewicz, Agnieszka
    Howard-Till, Rachel A.
    Novatchkova, Maria
    Mochizuki, Kazufumi
    Loidl, Josef
    CHROMOSOMA, 2010, 119 (05) : 505 - 518
  • [5] MRE11 and COM1/SAE2 are required for double-strand break repair and efficient chromosome pairing during meiosis of the protist Tetrahymena
    Agnieszka Lukaszewicz
    Rachel A. Howard-Till
    Maria Novatchkova
    Kazufumi Mochizuki
    Josef Loidl
    Chromosoma, 2010, 119 : 505 - 518
  • [6] Budding yeast Sae2 is an in vivo target of the Mec1 and Tel1 checkpoint kinases during meiosis
    Cartagena-Lirola, Hugo
    Guerini, Ilaria
    Viscardi, Valeria
    Lucchini, Giovanna
    Longhese, Maria Pia
    CELL CYCLE, 2006, 5 (14) : 1549 - 1559
  • [7] The plant-specific ABERRANT GAMETOGENESIS 1 gene is essential for meiosis in rice
    Chang, Zhenyi
    Xu, Chunjue
    Huang, Xiaoyan
    Yan, Wei
    Qiu, Shijun
    Yuan, Shuting
    Ni, Haoling
    Chen, Shujing
    Xie, Gang
    Chen, Zhufeng
    Wu, Jianxin
    Tang, Xiaoyan
    JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (01) : 204 - 218
  • [8] Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis
    Ding, ZJ
    Wang, T
    Chong, K
    Bai, SN
    SEXUAL PLANT REPRODUCTION, 2001, 13 (05): : 285 - 288
  • [9] Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis
    Zhao-jun Ding
    T. Wang
    Kang Chong
    Shunong Bai
    Sexual Plant Reproduction, 2001, 13 : 285 - 288
  • [10] A novel ATM-dependent X-ray-inducible gene is essential for both plant meiosis and gametogenesis
    Dean, Philip J.
    Siwiec, Tanja
    Waterworth, Wanda M.
    Schloegelhofer, Peter
    Armstrong, Susan J.
    West, Christopher E.
    PLANT JOURNAL, 2009, 58 (05): : 791 - 802