Resonance light scattering of catechol violet-cetyltrimethylammonium bromide-nucleic acid system and its analytical application

被引：0
|
作者：
Chen, YJ
Yang, JH [1 ]
Wu, X
Cao, W
Zhuang, HY
机构：
[1] Shandong Univ, Coll Chem & Chem Engn, Jinan 250100, Peoples R China
[2] Jinan Univ, Coll Chem & Chem Engn, Jinan 250002, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2003年 / 31卷 / 11期
关键词：
resonance light scattering; nucleic acid; catechol violet; cetyltrimethylammonium bromide;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper reports the spectral characteristic of resonance light scattering (RLS) system of catechol violet (PV)-cetyltrimethylammonium bromide (CTMAB)-nucleic acid. At pH 2.35, nucleic acid and CTMAB can cooperatively enhance the RLS intensity of PV and produce RLS with the maximum scattering peak located at 400 run. The enhanced RLS intensity is proportional to the nucleic acid concentration. Based on this phenomenon, ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) can be determined. The linear ranges of yRNA and fsDNA are both 20similar to750 mug/L, the limits of detection are 1.4 mug/L yRNA and 8.7 mug/L fsDNA. A new method for determination of nucleic acid was established.
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页码：1352 / 1355
页数：4
相关论文
共 9 条
  • [1] AGLISTER J, 1983, J CHEM PHYS, V78, P5358
  • [2] Chen Y J, 2002, CHINESE J SPECTROSCO, V19, P299
  • [3] Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP
    Chen, YJ
    Yang, JH
    Wu, X
    Wu, T
    Luan, YX
    [J]. TALANTA, 2002, 58 (05) : 869 - 874
  • [4] Spectrophotometry of nucleic acids by their effect on the complex of cobalt(II) with 4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene
    Huang, CZ
    Li, KA
    Tong, SY
    [J]. ANALYTICA CHIMICA ACTA, 1997, 345 (1-3) : 235 - 242
  • [5] Huang CZ, 1998, ANAL CHIM ACTA, V375, P89
  • [6] Determination of nucleic acids by a resonance light-scattering technique with alpha,beta,gamma,delta-tetrakis[4-(trimothylammoniumyl)phenyl]porphine
    Huang, CZ
    Li, KA
    Tong, SY
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (13) : 2259 - 2263
  • [7] Li Y F, 1998, CHINESE J ANAL CHEM, V26, P1508
  • [8] Study of the interaction of nucleic acids with acridine red and CTMAB by a resonance light scattering technique and determination of nucleic acids at nanogram levels
    Wang, M
    Yang, JH
    Wu, X
    Huang, F
    [J]. ANALYTICA CHIMICA ACTA, 2000, 422 (02) : 151 - 158
  • [9] Determination of nucleic acids using phosphin 3R as a fluorescence probe
    Zhu, QZ
    Yang, HH
    Li, DH
    Xu, JG
    [J]. ANALYTICA CHIMICA ACTA, 1999, 394 (2-3) : 177 - 184
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