Phylogenetic analysis of a G6P[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with G6P[1] and G10P[11] genotypes

被引:25
|
作者
da Silva Medeiros, Thais Neris [1 ]
Lorenzetti, Elis [1 ]
Alfieri, Alice Fernandes [1 ]
Alfieri, Amauri Alcindo [1 ]
机构
[1] Univ Estadual Londrina, Dept Vet Prevent Med, Lab Anim Virol, BR-86057970 Londrina, PR, Brazil
关键词
GROUP-A ROTAVIRUS; POLYMERASE-CHAIN-REACTION; MOLECULAR CHARACTERIZATION; P-TYPES; SEQUENCE-ANALYSIS; RISK-FACTORS; CALVES BORN; DAIRY HERDS; G6; IDENTIFICATION;
D O I
10.1007/s00705-014-2271-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim of this study was to perform the molecular characterization of the eleven genes of a G6P[5] bovine group A rotavirus (RVA) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. The outbreak affected 80 % of calves between 15-30 days old. RVA was identified by RT-PCR in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. The rotavirus wild-type strain had the genotype constellation G6(IV)-P[5](IX)-I2c-R2-C2-M2-A3-N2-T6-E2e-H3a. This study confirms the importance of homotypic immunity against the bovine RVA P[5] genotype in neonatal diarrhea in cattle herds that are regularly vaccinated against rotaviruses.
引用
收藏
页码:447 / 451
页数:5
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