Aptamer-modified magnetic SERS substrate for label-based determination of cardiac troponin I

被引：28

作者：

Lin, Chubing ^{[1
,2
]}

Li, Lijun ^{[1
,2
]}

Feng, Jun ^{[1
,2
]}

Zhang, Yan ^{[1
,2
]}

Lin, Xin ^{[1
,2
]}

Guo, Heyuanxi ^{[1
,2
]}

Li, Rui ^{[1
,2
]}

机构：

[1] Guangxi Univ Sci & Technol, Guangxi Key Lab Green Proc Sugar Resources, Coll Biol & Chem Engn, 268 Donghuan Rd, Liuzhou 545006, Guangxi, Peoples R China

[2] Prov & Minist Cosponsored Collaborat Innovat Ctr, Nanning 530004, Peoples R China

来源：

MICROCHIMICA ACTA | 2022年 / 189卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Surface-enhanced Raman scattering; Bimetallic nanoparticles; Coomassie Brilliant Blue; Label-based detection; Cardiac troponin I; AMPLIFICATION; NANOPARTICLES;

D O I：

10.1007/s00604-021-05121-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive label-based SERS strategy composed of magnetic bimetallic nanoparticles Fe3O4@Ag@Au, specific aptamer, and Bradford method was developed for the quantitative determination of cardiac troponin I (cTnI) in human serum. The prepared substrate with high magnetic character, signal enhancement, and uniformity exhibited significant Raman response. After the substrate was bound to the aptamer, the target protein cTnI was specifically captured, and it showed the Raman signal when the signal reporter Coomassie Brilliant Blue G-250 (CBBG) was supplied. The Raman signal intensity at 1621 cm(-1) showed a wide linear relationship with the log value of the cTnI concentration in the range 0.01 to 100 ng.mL(-1), and the estimated limit of detection (LOD) was 5.50 pg.mL(-1). The recovery and relative standard deviation (RSD) of the spike experiment in human serum samples were 92-115% and 7.4-12.7%, respectively.

引用

页数：8

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