PRODUCTION AND EVALUATION OF EXTRACELLULAR ENZYMES FROM BACILLUS LICHENIFORMIS IN DIFFERENT RAW MATERIALS USED IN ANIMAL FEED

The production of different enzymes, including amylase and protease by Bacillus licheniformis ATCC 21424 were tested on different raw materials and compound feed, which were used as a substrate in process fermentation: soybean meal, peas, sorghum flour, corn and compound feed (FC). The bacterial growth and enzyme production were done in a fermentative medium (Erlenmeyer 100 ml flash in a shaking incubator) and enzyme activity was registered after 24, 48 and 72 h, pH 7 +/- 2). The inoculum strain presents 10.19 log CFU/ml at 37 degrees C, 24 h, 150 rpm. The screening showed a capacity of amylase and protease strain production. The highest amylase activity was obtained when the strain was cultured in corn fermentation medium (19.43 U/ml), followed by soybean meal (18.31 U/ml), sorghum (17.52 U/ml), peas (19.43 U/ml), and FC (6.63 U/ml) at 72 h. Great protease activity was noticed in FC (97.75 U/ml), soybean meal (94.67 U/ml), sorghum (89.36 U/ml), corn (78.6 U/ml), peas respectively (75.91 U/ml). The observation of this study suggested that Bacillus licheniformis ATCC 21424 could be capable of producing protease and amylase enzymes, particularly in fermented medium contained soybean meal or corn, and can be administrated in animal nutrition as source of feed additive.