Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

被引:29
|
作者
Jiang, Yukun [1 ]
Guan, Yuzhe [1 ]
Lan, Yuanchen [1 ]
Chen, Shuo [1 ]
Li, Tiancheng [1 ]
Zou, Shujuan [1 ]
Hu, Zhiai [1 ]
Ye, Qingsong [2 ,3 ]
机构
[1] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China
[2] Wuhan Univ, Renmin Hosp, Ctr Regenerat Med, Wuhan, Peoples R China
[3] Harvard Med Sch, Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02115 USA
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Piezo1; bone remodeling; orthodontic tooth movement; alveolar bone; mechanotransduction; EXPRESSION; CHANNELS; RANKL;
D O I
10.3389/fphys.2021.767136
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.
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页数:10
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