Effect of ion transport inhibitors and methacholine on short-circuit current of isolated guinea pig nasal epithelium

被引:3
|
作者
Suzuki, K
Kawahara, K
Terada, N
Nomura, T
Konno, A
Fukuda, Y
机构
[1] Chiba Univ, Sch Med, Dept Physiol 2, Chuo Ku, Chiba 2608670, Japan
[2] Chiba Univ, Sch Med, Dept Otorhinolaryngol, Chuo Ku, Chiba 2608670, Japan
[3] Kitasato Univ, Sch Med, Dept Physiol, Sagamihara, Kanagawa 2280829, Japan
来源
JAPANESE JOURNAL OF PHYSIOLOGY | 1999年 / 49卷 / 01期
关键词
guinea pig nasal epithelium; Ussing chamber; short-circuit current; methacholine; ion transport inhibitors;
D O I
10.2170/jjphysiol.49.99
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To clarify the ion/water secretion mechanism in the nasal epithelial cells, the Ussing chamber method was applied to the nasal mucosa isolated from guinea pigs. The preparation, which contained surface epithelial cells, showed a small but consistent potential difference between mucosal and submucosal sides (mucosal surface negative to submucosa). The short-circuit current (l(sc)) across the epithelial layer was measured, and the effects of Na+ and/or Cl- transport inhibitors and methacholine (MCh) on l(sc) were analyzed. The basal l(sc) was almost totally suppressed by the combined application of amiloride (Na+ transport inhibitor) and low-Cl- Krebs Ringer (KR) solution or solutions containing Cl- transport inhibitors (furosemide or DPC). The application of MCh elicited triphasic l(sc) responses, i.e., initial transient increase (phase 1) followed by a small decrease (phase 2) and further sustained increase (phase 3) in l(sc). A possible ionic mechanism underlying phase 1 and 3 responses was analyzed. The Phase 1 response was greatly reduced by low-Cl- KR solution or furosemide but not influenced by amiloride. The Phase 3 response was augmented by amiloride and suppressed by low-Cl- KR solution, furosemide or DPC. These findings indicated that: the basal I,, was associated with Cl- secretion and/or Na+ absorption across epithelial cells under short-circuit condition and that MCh increased l(sc) probably via enhancing Cl- secretion in the nasal surface epithelial cell.
引用
收藏
页码:99 / 106
页数:8
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