Species identification by means of pyrosequencing the mitochondrial 12S rRNA gene

被引：57
|
作者：
Balitzki-Korte, B
Anslinger, K
Bartsch, C
Rolf, B
机构：
[1] Univ Hosp Giessen, Dept Legal Med, D-35392 Giessen, Germany
[2] Univ Munich, Dept Legal Med, Munich, Germany
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2005年 / 119卷 / 05期
关键词：
species identification; pyrosequencing; mitochondrial DNA; sequence analysis; 12S rRNA gene;
D O I：
10.1007/s00414-005-0537-9
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
The aim of this study was to develop a new method for species identification based on the analysis of a very short nucleotide sequence. For this reason, the mitochondrial 12S rRNA gene, together with the new method of pyrosequencing, was used. The detection of only 20 nucleotides, following the sequencing primer within a 149-bp fragment by pyrosequencing, was sufficient to identify the biological origin of the samples by alignment with a reference sequence database. A case example with a piece of skin is presented, and the question whether this piece of skin came from a missing wife or from an animal could be answered.
引用
收藏
页码：291 / 294
页数：4
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