Transcriptome analysis reveals differentially expressed small RNAs and genes associated with grapevine leafroll-associated virus 3 infections

被引：7
|
作者：
Bester, Rachelle [1 ]
Burger, Johan T. [1 ]
Maree, Hans J. [1 ,2 ]
机构：
[1] Stellenbosch Univ, Dept Genet, Private Bag X1, ZA-7602 Matieland, South Africa
[2] Infruitec Nietvoorbij Fruit Vine & Wine Inst, Agr Res Council, Private Bag X5026, ZA-7599 Stellenbosch, South Africa
来源：
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2017年 / 100卷
基金：
新加坡国家研究基金会;
关键词：
Grapevine leafroll disease; Next-generation sequencing; Plant-virus interaction; Virus-derived small interfering RNA; microRNA; ABIOTIC STRESS RESPONSES; ARABIDOPSIS-THALIANA; ANTIVIRAL DEFENSE; PLANT-GROWTH; RT-PCR; PROTEIN; ROLES; MICRORNAS; SEQUENCE; MIRNAS;
D O I：
10.1016/j.pmpp.2017.10.006
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The molecular interactions between grapevine leafroll-associated virus 3 (GLRaV-3) and three grapevine cultivars were characterised by identifying small RNAs and genes with differential expression between healthy and infected grapevine using next-generation sequencing and RT-qPCR assays. Cultivar specificity was identified in the sRNA and gene expression analyses, and both approaches identified Chenin blanc-specific responses providing the first insight into the differential symptom expression observed between cultivars. Differentially expressed genes identified in all three cultivars, provide a potential pool of genes displaying a molecular response to GLRaV-3 infection, contributing to the characterisation of this virus-plant interaction. (c) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：220 / 236
页数：17
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