Unveiling equal importance of two 14-3-3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen

被引:26
|
作者
Liu, Qian [1 ,2 ]
Li, Jin-Gen [2 ]
Ying, Sheng-Hua [1 ]
Wang, Juan-Juan [1 ]
Sun, Wen-Liang [2 ]
Tian, Chao-Guang [2 ]
Feng, Ming-Guang [1 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310058, Zhejiang, Peoples R China
[2] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
基金
中国国家自然科学基金;
关键词
ASPERGILLUS-NIDULANS; TRANSCRIPTION FACTOR; DNA-DAMAGE; ENTOMOPATHOGENIC FUNGUS; NUCLEAR-LOCALIZATION; GENE; EXPRESSION; GROWTH; ROLES; PHOSPHORYLATION;
D O I
10.1111/1462-2920.12634
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two conserved 14-3-3 proteins orthologous to Saccharomyces cerevisiaeBmh1/2 are poorly understood in filamentous fungi. Here we show that Bmh1 and Bmh2 contribute equally to the fundamental biology and physiology of Beauveria bassiana by targeting many sets of proteins/enzymes. Single Bmh deletion caused similar upregulation of another. Excellent knockdown (approximate to 91%) expressions of Bmh1 in Bmh2 and Bmh2 in Bmh1 resulted in equally more severe multiphenotypic defects than the single deletions, including G(2)/M transition, blastospore size, carbon/nitrogen utilization, conidiation, germination and conidial tolerances to high osmolarity, oxidation, cell wall stress, high temperature and UV-B irradiation. All the deletion and deletion/knockdown mutants showed similar defects in blastospore yield and density, hyphal septation and cell size, hyphal responses to most chemical stresses and virulence. All the defects were evident with altered transcripts of phenotype-related genes and well restored by each Bmh complementation. Our Bmh1- and Bmh2-specific transcriptomes generated under osmotic and oxidative stresses revealed up to 6% genes differentially expressed by at least twofold in the fungal genome. Many of those were greatly depressed or co-depressed in Bmh1 and Bmh2. Our findings provide a thorough insight into the functions and complementary effects of the two 14-3-3 proteins in the filamentous entomopathogen.
引用
收藏
页码:1444 / 1462
页数:19
相关论文
共 50 条
  • [1] 14-3-3 Proteins in Plant-Pathogen Interactions
    Lozano-Duran, Rosa
    Robatzek, Silke
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2015, 28 (05) : 511 - 518
  • [2] Calcium-activated 14-3-3 proteins as a molecular switch in salt stress tolerance
    Yang, Zhijia
    Wang, Chongwu
    Xue, Yuan
    Liu, Xiao
    Chen, She
    Song, ChunPeng
    Yang, Yongqing
    Guo, Yan
    [J]. NATURE COMMUNICATIONS, 2019, 10 (1)
  • [3] Calcium-activated 14-3-3 proteins as a molecular switch in salt stress tolerance
    Zhijia Yang
    Chongwu Wang
    Yuan Xue
    Xiao Liu
    She Chen
    ChunPeng Song
    Yongqing Yang
    Yan Guo
    [J]. Nature Communications, 10
  • [4] 14-3-3 proteins and the response to abiotic and biotic stress
    Roberts, MR
    Salinas, J
    Collinge, DB
    [J]. PLANT MOLECULAR BIOLOGY, 2002, 50 (06) : 1031 - 1039
  • [5] 14-3-3 proteins and the response to abiotic and biotic stress
    Michael R. Roberts
    Julio Salinas
    David B. Collinge
    [J]. Plant Molecular Biology, 2002, 50 : 1031 - 1039
  • [6] Stability and localization of 14-3-3 proteins are involved in salt tolerance in Arabidopsis
    Tan, Tinghong
    Cai, Jingqing
    Zhan, Erbao
    Yang, Yongqing
    Zhao, Jinfeng
    Guo, Yan
    Zhou, Huapeng
    [J]. PLANT MOLECULAR BIOLOGY, 2016, 92 (03) : 391 - 400
  • [7] Stability and localization of 14-3-3 proteins are involved in salt tolerance in Arabidopsis
    Tinghong Tan
    Jingqing Cai
    Erbao Zhan
    Yongqing Yang
    Jinfeng Zhao
    Yan Guo
    Huapeng Zhou
    [J]. Plant Molecular Biology, 2016, 92 : 391 - 400
  • [8] The 14-3-3 proteins: regulators of plant metabolism and stress responses
    Zhao, X.
    Li, F.
    Li, K.
    [J]. PLANT BIOLOGY, 2021, 23 (04) : 531 - 539
  • [9] 14-3-3 proteins: Macro-regulators with great potential for improving abiotic stress tolerance in plants
    Liu, Qing
    Zhang, Shaohong
    Liu, Bin
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 477 (01) : 9 - 13
  • [10] Overexpression of 14-3-3 proteins enhances cold tolerance and increases levels of stress-responsive proteins of Arabidopsis plants
    Visconti, Sabina
    D'Ambrosio, Chiara
    Fiorillo, Anna
    Arena, Simona
    Muzi, Carlo
    Zottini, Michela
    Aducci, Patrizia
    Marra, Mauro
    Scaloni, Andrea
    Camoni, Lorenzo
    [J]. PLANT SCIENCE, 2019, 289