Lactate Monitoring in Organotypic 3D Cell Cultures

被引：4

作者：

Hammer, S. ^{[1
]}

Weltin, A. ^{[1
]}

Kaminski, Y. ^{[2
]}

Noor, F. ^{[2
]}

Kieninger, J. ^{[1
]}

Urban, G. A. ^{[1
]}

机构：

[1] Univ Freiburg, Dept Microsyst Engn IMTEK, Lab Sensors, D-79110 Freiburg, Germany

[2] Univ Saarland, Biochem Engn Inst, D-66123 Saarbrucken, Germany

来源：

EUROSENSORS 2015 | 2015年 / 120卷

关键词：

Cancer; lactate; microsensors; electrochemical; monitoring; spheroids; toxicology; 3D cell culture;

D O I：

10.1016/j.proeng.2015.08.825

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology. (C) 2015 The Authors. Published by Elsevier Ltd.

引用

页码：961 / 964

页数：4

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