Growth suppression and cell death by pyridoxal is dependent on p53 in the human breast cancer cell line MCF-7

Vitamin B-6 compound, pyridoxine (PN), has shown antitumor action. Our previous experiments showed that PN induces expression of insulin-like growth factor binding protein-3 to arrest proliferation and induce cell death. This induction is inhibited by the p53-specific inhibitor pifithrin-alpha. Here, we report that another B-6 compound, pyridoxal (PL), strongly inhibited MCF-7 cell growth compared to PN. PL induced the G(0)/G(1) arrest and the accumulation of subG1 population. Although p53 mRNA was not changed by PL, 0.5mM PL increased the protein level in MCF-7 cells. The cell growth suppression by 0.5mM PL did not occur when p53 expression was knocked down using siRNA. Together, these data suggest that PL accumulate p53 and PL-induced cell growth suppression is dependent on p53 in MCF-7 breast cancer cells.