Lipid accumulation capability of typical non-acclimated activated sludge microbial consortia using methane gas as secondary carbon source

被引：0

作者：

Fortela, Dhan Lord B. ^{[1
,2
]}

Sharp, Wayne ^{[1
,3
]}

Revellame, Emmanuel ^{[1
,4
]}

Chistoserdov, Andrei ^{[5
]}

Holmes, William ^{[1
,2
]}

Gang, Daniel ^{[1
,3
]}

Hernandez, Rafael ^{[1
,2
]}

Zappi, Mark E. ^{[1
,2
]}

机构：

[1] Univ Louisiana, Energy Inst Louisiana, Lafayette, LA USA

[2] Univ Louisiana, Dept Chem Engn, Lafayette, LA USA

[3] Univ Louisiana, Dept Civil Engn, Lafayette, LA USA

[4] Univ Louisiana, Dept Ind Technol, Lafayette, LA USA

[5] Univ Louisiana, Dept Biol, Lafayette, LA USA

来源：

ENGINEERING REPORTS | 2020年 / 2卷 / 04期

关键词：

biofuels; biogas; bioprocess; lipids; methanotrophs; natural gas; METHYLOCYSTIS-HIRSUTA; BIOLOGICAL CONVERSION; NATURAL-GAS; FATTY-ACIDS; BIOGAS; BIODIESEL; IDENTIFICATION; METHANOTROPHS; PERSPECTIVES; BIOPRODUCTS;

D O I：

10.1002/eng2.12148

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

This work experimentally demonstrates that wastewater activated sludge microbial consortia can utilize methane resulting in lipid content enhancement. Activated sludge was cultivated using a synthetic wastewater as culture media. After initial purging with air, analytical grade methane gas was added in the culture headspace. The cultures were cultivated in batch-mode for 120hours at 25 degrees C in 500-mL bioreactors with 125-mL working liquid volume. Results indicate that methane gas was utilized by activated sludge microbial consortia under a similar pattern of microbial respiration as the control cultivated only with air. The activated sludge in the methane-purged bioreactors showed lipid enhancement (0.123 +/- 0.037mg lipid per mg biomass) and biomass growth (0.626 +/- 0.163mg biomass per mg glucose plus methane), which were higher than those in the control runs (0.009 +/- 0.034mg lipid per mg biomass and 0.225 +/- 0.133mg biomass per mg glucose).

引用

页数：9