Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

被引：6
|
作者：
Di Felice, Francesca [1 ]
Chiani, Francesco [1 ]
Camilloni, Giorgio [1 ,2 ]
机构：
[1] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy
[2] CNR, Ist Biol & Patol Mol, I-00185 Rome, Italy
来源：
BIOCHEMICAL JOURNAL | 2008年 / 409卷 / 03期
关键词：
chromatin; DNA structure; DNA topoisomerase I; nucleosome; Saccharomyces cerevisiae; trinucleotide repeats;
D O I：
10.1042/BJ20070893
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)(35), lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity.
引用
收藏
页码：651 / 656
页数：6
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