Mechanism of a Disassembly-Driven Sensing System Studied by Stopped-Flow Kinetics

被引：3
|
作者：
Gallo, Cara [1 ,2 ]
Thomas, Suma S. [1 ,2 ]
Selinger, Allison J. [1 ,2 ]
Hof, Fraser [1 ,2 ]
Bohne, Cornelia [1 ,2 ]
机构：
[1] Univ Victoria, Dept Chem, Victoria, BC V8W 2Y2, Canada
[2] Univ Victoria, Ctr Adv Mat & Related Technol CAMTEC, Victoria, BC V8W 2Y2, Canada
来源：
JOURNAL OF ORGANIC CHEMISTRY | 2021年 / 86卷 / 15期
基金：
加拿大自然科学与工程研究理事会;
关键词：
HOST; INCLUSION; DYNAMICS; BINDING; SENSOR; ASSAY;
D O I：
10.1021/acs.joc.1c00959
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We carried out steady-state and stopped-flow photo-physical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 assembly and nicotine binding were determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications. Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of the system.
引用
收藏
页码：10782 / 10787
页数：6
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