Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction

被引：12

作者：

Nakazato, Taro ^{[1
]}

Kawamura, Takuji ^{[1
]}

Uemura, Toshimasa ^{[2
,3
]}

Liu, Li ^{[1
]}

Li, Junjun ^{[1
]}

Sasai, Masao ^{[1
]}

Harada, Akima ^{[1
]}

Ito, Emiko ^{[1
]}

Iseoka, Hiroko ^{[1
]}

Toda, Koichi ^{[1
]}

Sawa, Yoshiki ^{[1
]}

Miyagawa, Shigeru ^{[1
]}

机构：

[1] Osaka Univ, Dept Cardiovasc Surg, Grad Sch Med, Suita, Osaka, Japan

[2] Osaka Univ, Dept Precise & Sci Technol, Grad Sch Engn, Suita, Osaka, Japan

[3] JTEC Corp, Cell Culture Mkt & Res Ctr, Ibaraki, Osaka, Japan

来源：

STEM CELL REPORTS | 2022年 / 17卷 / 05期

关键词：

CELL-DERIVED CARDIOMYOCYTES; FUNCTIONAL MATURATION; DYNAMIC CULTURE; SHEETS; SURVIVAL; CONTRACTION; ACTIVATION; ADHESION; MUSCLE; FORCE;

D O I：

10.1016/j.stemcr.2022.03.012

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3DhiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure.

引用

页码：1170 / 1182

页数：13

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