Black pepper (Piper nigrum L.) associated endophytic Pseudomonas putida BP25 alters root phenotype and induces defense in rice (Oryza sativa L.) against blast disease incited by Magnaporthe oryzae

被引:41
|
作者
Ashajyothi, Mushineni [1 ]
Kumar, Aundy [1 ]
Sheoran, Neelam [1 ]
Ganesan, Prakash [1 ]
Gogoi, Robin [1 ]
Subbaiyan, Gopala Krishnan [2 ]
Bhattacharya, Ramcharan [3 ]
机构
[1] ICAR Indian Agr Res Inst, Div Plant Pathol, New Delhi 110012, India
[2] ICAR Indian Agr Res Inst, Div Genet, New Delhi 110012, India
[3] Natl Inst Plant Biotechnol, New Delhi 110012, India
关键词
Pseudomonas putida; Endophytism; Rice; M; oryzae; Induced defense; Blast disease; Seed priming; SYSTEMIC RESISTANCE; SEED TREATMENT; GENES; COLONIZATION; INFECTION; RESPONSES; IDENTIFICATION; MONOOXYGENASE; DETERMINANTS; SUPPRESSION;
D O I
10.1016/j.biocontrol.2019.104181
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Blast disease caused by Magnaporthe oryzae is an important production constraint in rice farming worldwide. Novel strategies are needed to combat blast disease as the current options like host resistance and fungicides are either unstable or not-acceptable. Black pepper associated Pseudomonas putida BP25 endogenously colonized rice-a monocot, in a density-dependent manner with altered root growth and defense activation, also showed bio-control activity against blast caused by M. oryzae. Fluorescence imaging of P. putida BP25 primed seedlings revealed extensive bacterial colonization in roots that was further confirmed in plate assay and qPCR. Although initial counts could be correlated with bacterial dose used for priming, the bacterial population stabilized 14-days post priming suggesting internal regulation of endophyte colonization. Endophytic colonization of rice triggered alteration in root morphology, possibly, due trade-off between growth and defense. P. putida BP25 significantly inhibited the mycelium of M. oryzae by both volatile and non-volatile metabolites and also seed priming protected the rice from blast. Activities of defense related peroxidase and phenols were found increased in primed plants. qPCR assay of rice defense and developmental genes indicated increased expression of Systemic Acquired Resistance (SAR) related OsPR1-1 (Pathogenesis Related protein 1-1) and down-regulation of OsPR3. In addition, rice seedlings emerged from endophyte priming showed down-regulation of stress responsive OsACO4 and OsACS6 involved in inter-nodal elongation which plays an important role in growth and development.
引用
收藏
页数:10
相关论文
共 11 条
  • [1] Antifungal and defense elicitor activities of pyrazines identified in endophytic Pseudomonas putida BP25 against fungal blast incited by Magnaporthe oryzae in rice
    Asharani Patel
    Aundy Kumar
    Neelam Sheoran
    Mukesh Kumar
    Kuleshwar Prasad Sahu
    Prakash Ganeshan
    Mushineni Ashajyothi
    Subbaiyan Gopalakrishnan
    Robin Gogoi
    [J]. Journal of Plant Diseases and Protection, 2021, 128 : 261 - 272
  • [2] Hydroxamate siderophores secreted by plant endophytic Pseudomonas putida elicit defense against blast disease in rice incited by Magnaporthe oryzae
    Ashajyothi, Mushineni
    Velmurugan, Shanmugam
    Kundu, Aditi
    Balamurugan, Alexander
    Chouhan, Vinod
    Kumar, Aundy
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2023, 76 (12)
  • [3] Differential biochemical response of rice (Oryza sativa L.) genotypes against rice blast (Magnaporthe oryzae)
    Anushree, P. U.
    Naik, R. M.
    Satbhai, R. D.
    Gaikwad, A. P.
    Nimbalkar, C. A.
    [J]. COGENT BIOLOGY, 2016, 2 (01):
  • [4] First evidence of rice (Oryza cf. sativa L.) and black pepper (Piper nigrum) in Roman Mursa, Croatia
    Reed, Kelly
    Lelekovic, Tino
    [J]. ARCHAEOLOGICAL AND ANTHROPOLOGICAL SCIENCES, 2019, 11 (01) : 271 - 278
  • [5] First evidence of rice (Oryza cf. sativa L.) and black pepper (Piper nigrum) in Roman Mursa, Croatia
    Kelly Reed
    Tino Leleković
    [J]. Archaeological and Anthropological Sciences, 2019, 11 : 271 - 278
  • [6] A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds
    Wang, Zhishan
    Wu, Xianyu
    Li, Ni
    Wang, Weiping
    Liu, Yang
    [J]. PLANT GROWTH REGULATION, 2022, 96 (02) : 237 - 241
  • [7] A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds
    Zhishan Wang
    Xianyu Wu
    Ni Li
    Weiping Wang
    Yang Liu
    [J]. Plant Growth Regulation, 2022, 96 : 237 - 241
  • [8] Riboflavin Induces Different Defense Responses against Pyricularia oryzae in Improved and Traditional Rice (Oryza sativa L.) Cultivars
    Aghajanzadeh, T. A.
    Jazayeri, O.
    [J]. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY, 2018, 20 (05): : 1071 - 1082
  • [9] Endophytic bacterial flora in root and stem tissues of black pepper (Piper nigrum L.) genotype: isolation, identification and evaluation against Phytophthora capsici
    Aravind, R.
    Kumar, A.
    Eapen, S. J.
    Ramana, K. V.
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2009, 48 (01) : 58 - 64
  • [10] Development of pyramided lines with two resistance genes, Pish and Pib, for blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.)
    Koide, Y.
    Kawasaki, A.
    Telebanco-Yanoria, M. J.
    Hairmansis, A.
    Nguyet, N. T. M.
    Bigirimana, J.
    Fujita, D.
    Kobayashi, N.
    Fukuta, Y.
    [J]. PLANT BREEDING, 2010, 129 (06) : 670 - 675