INSIGHT INTO STREPTOMYCES GRISEORUBENS JS']JSD-1: MOLECULAR MECHANISM OF LIGNOCELLULOSE DEGRADATION

Biodegradation of rice straw was carried out using a actinomycete isolate, identified as Streptomyces griseorubens JSD-1 that was able to produced varieties of lignocellulolytic enzymes. Consequently, it could metabolize 88% rice straw within ten days incubation and the enzymatic abilities were also determined when induced by different carbon sources. To demonstrate the molecular mechanism of biodegradation, genome sequencing was carried out by Miseq platform and finally a draft genome map of JSD-1 with high GC content of 72.42% was obtained. Thereafter, genes related were acquired for the following characterization of their cellular localizations. Through the analysis of quantitative real-time PCR, these genes were proved to be related to the metabolism of lignocellulose as their expression were generally and significantly up-regulated with the induction of rice straw. Finally, heterogeneous expression of their recombinant proteins were also optimized for efficient production. In conclusion, our findings provided the genetic or genomic background of utilizing lignocellulose in this isolate.